Simultaneous assessment of cardiac metabolism and perfusion using copolarized [1-13C]pyruvate and 13C-urea


Purpose: To demonstrate the feasibility of imaging a bolus of co-polarized [1-13C]pyruvate and 13C-urea to simultaneously assess both metabolism and perfusion in the rodent heart. Methods: Co-polarized [1-13C]pyruvate and 13C-urea was imaged using a multi-echo, flow-sensitized spiral pulse sequence. Healthy rats were scanned in a two-factor factorial design (nā€‰=ā€‰12 total; metabolism: overnight fasting versus fed with dichloroacetate injection; perfusion: rest versus adenosine stress-induced hyperemia). Results: Alterations in metabolism were detected by changes in pyruvate metabolism into 13C-bicarbonate. Statistically independent alterations in perfusion were detected by changes in myocardial pyruvate and urea signals. Conclusion: The new pulse sequence was used to obtain maps of metabolism and perfusion in the rodent heart in a single acquisition. This hyperpolarized 13C imaging test is expected to enable new studies in which the cardiac metabolism/perfusion mismatch can be studied in the acute environment.

In Magnetic Resonance in Medicine